Frequência dos alelos HLA A, B e DRB1 em doadores voluntários de medula óssea do estado de Mato Grosso do Sul no período de 2012 a 2013

Abstract

The Brazilian population presents great racial miscegenation and it has originated primarily, from the Caucasians of European origin, Africans and Indians. The Brazil's largest population group is understood by mixed race people originated from the miscegenation occurred between different races. Therefore, the investigation of the frequency of HLA antigens and their allelic diversity, held in the state of Mato Grosso do Sul, it is extremely relevant enabling the improvement of routine transplants in our state. This study aimed to investigate the frequency of HLA antigens and their allelic diversity from 2012 to 2013. The results of HLA typing were obtained from secondary data from the sample database processed by the Molecular Biology Laboratory and Histocompatibility Ltda. (Biomolecular Laboratory), collected in partnership with the "José Scaff" Center of Hematology and Hemotherapy of Mato Grosso do Sul (MS-HEMOSUL). The allele determinations were made using the kits LABType PCR-SSO One Lambda. The data were placed in an Excel spreadsheet, which was then processed by two softwares, IBM SPSS Statistics 22 (SPSS - StatisticalPackage for Social Sciences) and Arlequin. It was randomly selected 10% of the 16,430 samples analyzed in the period, and the study had 1,643 samples. The subjects were categorized into: White people (n = 999; 60.8%), Brown people (n = 421; 25.6%), Yellow people (n = 24; 1.4%), Black people (n = 86; 5, 2%), Indigenous (n = 11; 0.7%) and 102 (6.3%) patients who did not declare their race. They were also categorized by regions of Mato Grosso do Sul: Central - north (n = 366; 22.3%), East (n = 497; 30.2%), Pantanal (n = 281; 17.1%) and Southwest (n = 499; 30.4%). The HLA A, B alleles and common DRB1 were A * 02 (24.4%) B * 35 (11.7%) and DRB1 * 04 (14.0%). No statistically significant differences were observed regarding the heterozygosity, and also on the results of Hardy-Weinberg test.