Terapia com células tronco mesenquimais promove melhorias e reestabelecimento das funções renais em modelo pré-clínico

Abstract

Objective: Examine the effects of bone mesenchymal stem-cells injection in the renal function of a rat model submitted to cisplatin-induced acute kidney injury (AKI). Methods: In order to investigate the effects of bone mesenchymal stem-cells injection (MB-MSCs) in a cisplatin-induced AKI rat model (intraperitoneal injection of 5 mg cisplatine/kg body weight; Fauldcispla LIBBS, Batch 12 G0702). Fifty-nine rats were divided into two main groups: donor (n=10) and experimental (n=49). The first group received a single intraperitoneal injection (IP) of saline and the MB-MSCs were extracted from them. The experimental group was subdivided into seven subgroups, each one containing seven rats: subgroups R-I, R-IV and R-V received single dose of IP cisplatin and were evaluated at days 2, 30 and 45, respectively. Subgroups R-II and R-III received IP cisplatin plus BM-MSCs injection and were evaluated at days 30 and 45, respectively; subgroups R-VI and R-VII were included as controls. Quantification of renal function was performed by serum analysis of BUN and creatinine. Evaluation of the histological damage by optical microscopy. Statistical analysis was performed and a p value < 0,05 was considered significant. Results: Significant improvement of the renal function was observed with reduction of creatinine at days 30 (p = 0,002) and 45 (p < 0,001), along with BUN reduction at days 30 (p= 0<002) and 45 (p < 0,001). Improvement of histological lesions MSCs was observed at day 30 (intra-tubular protein, p=0,004) and at day 45 (intratubular protein, p=0,004; tubular dropsical degeneration, p < 0,001). Conclusion: Administration of BM-MSCs stem cells (bone marrow stromal cells) for renal repair after cisplatine-induced acute kidney injury in rats showed a significant improvement of renal function and histological lesions.