Use este identificador para citar ou linkar para este item: https://repositorio.ufms.br/handle/123456789/1252
Tipo: Artigo de Periódico
Título: Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil
Autor(es): Rodrigues, Ana Claudia Souza
Chang, Marilene Rodrigues
Nóbrega, Gabriela Dorn
Rodrigues, Mayara Souza
Carvalho, Nadia Cristina Pereira
Gomes, Beatriz Garcia
Almeida, Dênio Lopes de
Carvalho, Karyne Rangel
Asensi, Marise Dutra
Abstract: Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.
Palavras-chave: Pseudomonas Aeruginosa
Resistência a Medicamentos
Beta-Lactamases
Eletroforese
Eletroforese em Gel de Campo Pulsado
Drug Resistance
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Editor: Brazilian Journal of Infectious Diseases
Citação: RODRIGUES, Ana Claudia Souza et al . Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil. Braz J Infect Dis, Salvador, v. 15, n. 3, June 2011 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000300002&lng=en&nrm=iso>. access on 17 Apr. 2012.
Tipo de Acesso: Acesso Aberto
Identificador DOI: http://dx.doi.org/10.1590/S1413-86702011000300002.
URI: https://repositorio.ufms.br/handle/123456789/1252
Data do documento: Mai-2011
Aparece nas coleções:CCBS - Artigos publicados em periódicos

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