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dc.contributor.authorRodrigues, Ana Claudia Souza-
dc.contributor.authorChang, Marilene Rodrigues-
dc.contributor.authorNóbrega, Gabriela Dorn-
dc.contributor.authorRodrigues, Mayara Souza-
dc.contributor.authorCarvalho, Nadia Cristina Pereira-
dc.contributor.authorGomes, Beatriz Garcia-
dc.contributor.authorAlmeida, Dênio Lopes de-
dc.contributor.authorCarvalho, Karyne Rangel-
dc.contributor.authorAsensi, Marise Dutra-
dc.identifier.citationRODRIGUES, Ana Claudia Souza et al . Metallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazil. Braz J Infect Dis, Salvador, v. 15, n. 3, June 2011 . Available from <>. access on 17 Apr. 2012.pt_BR
dc.description.abstractInfection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State, from January 1st, 2007 to June 31st, 2008. Profiles of antimicrobial susceptibility were determined using the agar diffusion method. Metallo-β-lactamase was investigated using the double-disk diffusion test and PCR. Molecular typing was performed by pulsed-field gel electrophoresis (PFGE). Respiratory and urinary tracts were the most common isolation sites. Of the 40 samples tested, 72.5% (29/40) were resistant to ceftazidime and 92.5% (37/40) to imipenem, whereas 65% (26/40) were resistant to both antimicrobials. Fifteen pan-resistant samples were found. Five percent (2/40) of samples were positive for metallo-β-lactamase on the phenotype test. No metallo-β-lactamase subtype was detected by PCR. Macrorestriction analysis revealed 14 distinct genetic patterns. Based on the superior accuracy of PCR, it can be inferred that P. aeruginosa isolates from the investigated hospitals have alternative mechanisms of carbapenem resistance. The results also suggest clonal spread of P. aeruginosa between the studied hospitals.pt_BR
dc.publisherBrazilian Journal of Infectious Diseasespt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectPseudomonas Aeruginosapt_BR
dc.subjectResistência a Medicamentospt_BR
dc.subjectEletroforese em Gel de Campo Pulsadopt_BR
dc.subjectDrug Resistancept_BR
dc.subjectElectrophoresis, Gel, Pulsed-Fieldpt_BR
dc.titleMetallo-β-lactamase and genetic diversity of Pseudomonas aeruginosa in intensive care units in Campo Grande, MS, Brazilpt_BR
dc.typeArtigo de Periódicopt_BR
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