Caracterização e avaliação in vitro da atividade antibacteriana de vidro bioativo dopado com própolis verde de apis mellifera

Abstract

The bioceramics have applications in dentistry because of its exceptional biological properties. The bioactive glass (BG) has been widely studied and usedin Periodontics, with antimicrobial activity, biocompatibility and osteoconductive function. The ability of the bioactive glass to be antimicrobial allowed its association with propolis, a product derived from the bees (Apis mellifera), with proven antimicrobial and anti-inflammatory efficacy. This study aimed to incorporate the bioactive glass in 1:10, extracts of green propolis (EGP) at 30%, fractionated by liquid-liquid partition in different vehicles (hexane, ethyl acetate, hydroalcoholic). It has been performed the physical characterization and evaluation of their antimicrobial potential, through tests of Minimum inhibitory concentration (MIC) and death curve technique against pour plate Escherischia coli (E. coli) ATCC 25922 and Staphylococcus aureus (S. aureus) ATCC 25923. It has been conducted in vitro periodontal biofilm formation on polystyrene plate and evaluated their potential to inhibit growth. The chemical results showed that propolis has a large amount of phenolic compounds such as flavonoids. This was verified by physical characterization by Differential Scanning Calorimetry (DSC), which verified the presence of different substances in each of the fractions of EGP. In MIC tests, all fractions showed antimicrobial activity on bacteria tested. The BG was efficient as a bactericide, as well as the association BG /EGP. Regarding the biofilm, promoted its elimination, which did not occur in the association BG / EGP. It is possible to associate the green propolis extract the BG, but it loses its ability to destroy the biofilm activity, possibly due to neutralization reaction, maintaining the antimicrobial activity. Other in vitro tests are needed to ensure its use in vivo.