Análise histopatológica e morfométrica nuclear de hepatócitos de Danio rerio (zebrafish) expostos à água de córregos urbanos, Campo Grande, MS

Abstract

Aquatic biota is constantly facing increasing exposure to toxic substances from human activities, which have caused changes in the physical-chemical and biological processes of water sources. These changes, in turn, contribute to the degradation of environmental quality, putting the health of the organisms that inhabit these ecosystems at risk. In this context, the use of aquatic organisms as bioindicators makes it possible to estimate the effects and level of contamination in aquatic environments. In order to evaluate the impacts of water from three urban streams in the city of Campo Grande, in the state of Mato Grosso do Sul, a study was conducted using the Danio rerio fish as an environmental indicator. For this purpose, active biomonitoring was adopted, and the specimens were subjected to exposures of 3, 6 and 12 days. The results obtained revealed that the specimens exposed to stream water presented tissue injuries of low to high degrees of irreversibility. Furthermore, differences were observed in hepatocyte nuclear volume as well as integrated optical density (DOI). Based on these findings, we can conclude that the water from the three streams changed the organization and structure of hepatocytes, showing different profiles according to the exposure time. This biomonitoring approach can be applied in future studies involving other aquatic environments, improving the model's laboratory techniques and providing support for the development of new conservation and environmental monitoring strategies.