Atividade antimicrobiana in vitro de seis cimentos endodônticos avaliados em dois métodos

Abstract

Objective: The present study evaluated the antimicrobial activity of sealers Endofill®, Acroseal®, Sealer 26®, Sealapex®, Endomethasone N® and AH plus® front the bacteria Enterococcus faecalis, Escherichia coli, Staphylococcus aureus and the yeast Candida albicans. Method: For the antimicrobial tests, was used two different methods: ágar diffusion test and direct contact in liquid. For the diffusion test in ágar wells were prepared, the cements were inserted and stored in the bacteriological incubator at 37 ° C for 48 hours. After this period were measured the inhibition of microbial through a digital caliper. In direct contact test, the sealers were placed in test tubes containing broth TSB, passed the 20 minutes after, the microorganism were added and reading was performed by measuring the absorbance of each grade cement using a spectrophotometer, in times of 24 and 48 hours. Was carried out, for each strain, triplicate in ágar diffusion tests, and duplicate tests in direct contact in a liquid medium. For analysis of results, was performed statistical tests of Kruskal-Wallis and Student-Newman-Keuls. Results: Within the results can be observed that in the ágar diffusion test all sealers showed antimicrobial activity against some microorganisms, and sealers based on zinc oxide and eugenol had better antimicrobial capabilities compared to others, detaching the Endomethasone N® that demonstrated inhibition zones larger than the rest of the materials front the bacteria S. aureus and E. coli faecalis with no significant differences with the positive control groups, which were respectively: Vancomycin, Chloramphenicol and Ampicillin (p> 0,05). In direct contact test, the Endomethasone N® and Endofill® also had lower levels of absorbance, demonstrating high antimicrobial activity, with no significant difference between the materials and the positive control groups mentioned above by adding the antimicrobial Ketoconazole (positive control), used for testing against C. albicans (p> 0,05). Conclusion: Given the above it was concluded that the sealers based on zinc oxide and eugenol showed higher antibacterial activity within 48 hours, against the tested microorganisms in the two methods mentioned, highlighting the material Endomethasone N®.