Estudo histológico da ação do plasma rico em plaquetas, associado ou não ao laser de baixa potência, na cicatrização por primeira intenção, em ratos Wistar

Abstract

The aim of this study was to evaluate the biological behavior of Platelet-Rich Plasma (PRP) associated or not to low intensity laser in first intention healing of experimentally induced wounds on cutaneous tissue of rats. 24 adult female Wistar rats were divided in four groups: control (C), PRP (P), laser (L) and PRP associated to laser (PL). During the observation period the groups were subdivided in three subgroups with two animals each, observed by 7, 17 and 21 days post-surgery. A lesion on the thigh was made with a punch and filled with PRP gel in the groups P and PL. In the groups C and L the les ion was filled with blood. The laser therapy was performed in a punctual way at 0, 1, 2 and 3 days post-surgery, with a total daily dose of 20 J/ cm² in the groups P and PL. Tissue sections were stained by hematoxylineosin and Masson’s trichromic and submitted to semi-quantitative histological analysis to evaluate the number of leucocytes and the collagenation and reepitelization degrees. The results suggest that the proliferation of fibroblasts and the presence of collagen fibers at day 7 were greater in the group PL when compared to the other groups. This study showed evidences of the benefic effect of PRP associated to laser on wound healing, but further studies are needed.