Ação antimicrobiana e funcional de méis do estado de Mato Grosso do Sul

Abstract

Honey is a food of complex composition very appreciated by man since ancient times. The aim of this work was to evaluate honeys produced in the State of Mato Grosso do Sul. Honeys were obtained in plastic polyethylene packages ready for commercialization of the monofloral types of grape vine and aroeira and polifloral honey from Apiário Vovô Pedro, in Campo Grande/MS, and polifloral honey benefited in Bodoquena / MS from AgroHB. Three samples of each type of honey were sent to the Food Technology and Public Health Unit (UTASP / CCBS) of the UFMS, placed in hygienized styrofoam boxe stored in a room with a refrigerated temperature circa 20°C, far from source. direct light, where physical-chemical analysis of honey identity and quality of Apis mellifera (BRASIL, 2000), evaluation of the antioxidant potential and content of bioactive compounds (total phenols, tannins and vitamin C), determination of the coloration, pollen analysis, and evaluation of the antimicrobial activity with the use of the strains of Staphylococcus aureus, Escherichia coli and Salmonella spp ., For observation of the minimum inhibitory concentration (MIC) and minimum bacterial (CBM). The identity and quality analyzes performed on honey presented 100% compliance in all parameters, attesting to the good quality of the evaluated honey, which is a result of effective production processes and compliance with the legislation in force with the consumer. The samples presented color classification as extra-light amber for the samples of honey of grape-vine and light-amber for the others. Pollen analysis revealed a disagreement between the pollen types found in the samples and the information on the labels, with a predominance of the Schinus terebinthifolius floral types., red aroeira, and Myracrodruon urundeuva, black aroeira. The antioxidant potential and bioactive content found in the samples were considered relevant because they presented values that were sometimes higher than those found in the literature, however the polifloral declared honeys were higher statically. The antimicrobial activity of the honeys againist strains of S. aureus, E. coli and Salmonella spp. It was obtained after bacterial formation and subsequent deep inoculation of the results in significant inhibitions, with inhibition halos with performance close to the antibiotic of choice (azithromycin) for E. coli and Salmonella sp. S. aureus presented higher resistance to the honeys of the study. Most of the samples had only inhibitory effect against the strains of the study, with MIC ranging from 0.795 ± 0.013 to 0.098 ± 0.001 mg mL-1 and only the samples of declared of grapevine and Campo Grande polifloral against E. Coli and polifloral from Bodoquena against Salmonella spp. Presented MBC at concentrations of 0.394 ± 0.006, 1.563 ± 0.003 and 0.382 ± 0.005 mg mL-1 respectively. Therefore, it is possible to observe an interesting antioxidant and antimicrobial activity in the samples, where there are indications of the presence of compounds with such characteristics, having a great potential of use of Apis mellifera honeys produced in Mato Grosso do Sul.