Influência da suplementação com creatina sobre o perfil metabólico sistêmico e marcadores de remodelação do miocárdio de ratos submetidos a exercício físico resistido

Abstract

INTRODUCTION: Several studies have showed different ergogenic effects of the creatine supplementation when associated with exercise in most variated tissues; however, some evidence have suggested that creatine intervention has been associated to glycemic metabolic disorders. Additionally, its influence on the cardiac muscle is still little studied. OBJECTIVE: To analyze the influence of creatine supplementation on the systemic metabolic profile and myocardial remodeling in rats submitted to resistive physical exercise. MATERIALS AND METHODS: Wistar rats (n = 60) were randomized into four groups: Control (C), Creatine (CR), Physical Exercise (EF), and Exercise plus Creatine (EFCR). While C and EF groups were treated with standard commercial feed (3.61 Kcal / g) ad libitum, CR and EFR groups received commercial chow supplemented with 2% of creatine monohydrate (3.68 Kcal / g). Moreover, EF and EFCR were also submitted to a resisted physical exercise protocol of climbing, three times a week. After 12 weeks, nutritional and murinometric behavior, functional capacity, serum biochemical profile, as well as macroscopic and microscopic morphology of the heart were analyzed. Nutritional, murinometric and glycemic tolerance variables were evaluated by Two-Way ANOVA in the repeated measures model, followed by the Bonferroni multiple comparisons test. Other variables were evaluated using Two-Way ANOVA, complemented with Tukey's test. All statistical conclusions were discussed under the significance level of 5%. RESULTS: Rats submitted to resistive exercise presented higher functional capacity. Exercise, when associated with creatine supplementation, reduced glycemic tolerance. In the cardiac context, EFCR group showed higher values of mass of the heart, atrium and left ventricle, when normalized by the final body mass. CONCLUSION: Creatine supplementation associated with resistive exercise promoted glycemic metabolic disorders. In the morphological context, exercise associated with creatine supplementation resulted cardiac remodeling.