Análise da influência da aplicação do laser de baixa potência sobre a ação das proteínas morfogênicas ósseas (BMP) em defeitos ósseos provocados em ratos wistar

Abstract

Bone morphogenetic proteins (BMPs) are secreted signaling molecules belonging to the transforming growth factor-ß (TGF-ß) superfamily. The objective of this study was to analyze how GaAlAs 650nm laser influenced BMPs action on bone defects created in rat femurs. The sample consisted of 36 male albino Wistar rats − Group 1: (n=12) Bone defect filled with bone-inducing substance, with application of low-power laser; Group 2: (n=12) Bone defect filled with bone-inducing substance, without application of low-power laser and Group 3: (n=12) Bone defect without any treatment (control group). A bone defect was produced using drills. In Groups 1 and 2 defects were filled with a bone-inducing substance. Animals were treated with GaAlAs (50mW) laser, energy density 4 J/cm2, for 80 seconds on a 1 cm2 irradiated area. Groups 3 were used as control. Bone samples were removed for histological procedures and morphometric analysis on the 7th, 14th and 28st days after surgery. Results obtained were subjected to statistical analysis using Kruskal-Wallis post hoc test de Dunns. Rejection level for the nullity hypothesis was 0,05. On the comparison among G1, G2 and G3 could be observed statistical difference between Groups 1 and 3 (p < 0.001). We concluded that the association of low power laser with a boneinducing substance produced better results than when low power laser or BMPs used alone.