Ação da própolis de Apis mellifera associada ao fluoreto de sódio no processo de remineralização de manchas brancas e sobre a microbiota cariogênica : estudo in situ

Abstract

The association of propolis to fluoride has presented promising results on in vitro and in vivo essays for caries control. The objective of the present study was to verify the effect of a 5% propolis ethanol extract (EEP 5%) associated to 0.05% sodium fluoride (NaF 0.05%) on the cariogenic dental biofilm and the demineralization-remineralization processes of the enamel. A 2-phase, 14 days each, in situ study was performed; on Phase 1, the volunteers wore palatal devices containing 6 enamel blocks, which were submitted to cariogenic challenge (CC) with 20% sucrose 8 times a day. During Phase 2, volunteers were divided in 5 experimental groups treated as follows: (1) CC+control; (2) CC+NaF 0.05%; (3) CC+EEP 5%; (4) CC+EEP 5% + NaF 0.05% and (5) CC + acidulated phosphate fluoride. At the end of each phase, the microbiologic analysis of biofilm and microhardness of transversal sections of the enamel were performed. Group 4 presented statistically significant differences when compared to the other studied groups and among the phases, regarding mineral gain and microhardness recover, as well as bacteria supression. It could be concluded that the association of EEP 5%+NaF 0.05% was effective in controlling cariogenic biofilm and mineral reequilibrium.