Efeito osteogênico do laser de baixa potência aliado a enxerto ósseo no tratamento de defeito produzido no fêmur de ratas ovariectomizadas

Abstract

A bone fracture may represent potential bone-muscle alterations and months of hospitalization, leading to grave health issues. There are cases where the osteogenesis process may be accelerated. The aim of the research was to analyze the osteogenic effect of low-level laser in union with bone and absorbable cortical bone membrane grafts in treating defects produced in the femur of female mice submitted to ovariectomy. Bilateral ovariectomy was performed in 108 female (adult) mice of Wistar lineage and after the evidence of reduced bone mass, done through bone density digital analysis; a standard perforation was made on the femur’s diaphysis (2mm). The animals divided in groups were treated with As-Ga-Al Laser (660nm); In-Ga-Al-P Laser (808nm) as well as bone and bovine cortical bone membrane grafts. The animals underwent euthanasia after 7, 14 and 28 days. The sections destined to histology were colored with HE for bone cell analysis: bone Trabeculae, Osteoblasts, Osteocytes, Fibroblasts and Collagen. Histological descriptive analysis and morphometric evaluation to quantify the representative areas of neoformed bone were also performed. The histological analysis, when analyzing bone trabeculae, showed that the animals treated with In-Ga-Al-P Laser (808nm) presented better results after 28 days when compared to the As-Ga-Al Laser (660nm) group results. allowing for the identification of ideal laser therapy parameters to induce the increase of cells responsible for bone growth and repair.