Caracterização de um modelo animal de doença renal produzida por isquemia e reperfusão, em Rattus norvegicus WISTAR

Abstract

In order to characterize an animal model of kidney disease, they were used eight male and eight female rats (Rattus norvegicus) of the Wistar strain, between 60 and 81 days of age, who underwent surgery for clamping the renal pedicle ( artery and vein) unilateral left for 10 minutes to ischemia with restoration of flow to reperfusion. Biomarkers were evaluated: urinary alkaline phosphatase activity (FAU) and gamma glutamyltransferase (gGTU); urinary glucose (GliU); Urinary protein (PRV); urinary creatinine (CRU); relative urinary protein and urinary creatinine (PRU: Raw) at 24 hours, at 5, 7, 14 and 21 days. It was observed that the unilateral ischemia for 10 min showed the evaluation of urine at 24 hours, a significant increase in enzymatic activity of gGTU and FAU, besides increasing the GliU, which indicates acute tubular injury. These enzymes showed a decrease of their values, after 24 hours, and equated to the control group values from five to seven days. From the fifth day it was observed the appearance of protein (albumin) in both the urine type I and in spectrophotometry. Seven days in sediment assessment also appeared granular cylinders. Both the protein and granular cylinders remained in urine until the 21st day and, together with glucosuria in descent values demonstrated that cronificou injury, as seen at 21 days, and can evolve into chronic tubular injury. The model of ischemia for 10 minutes proved feasible, because it was characterized by changes in urinary biochemical parameters analyzed, and the evolution of the disease.