Efeito do exercício físico aeróbico sobre os níveis de estresse oxidativo em camundongos

Abstract

Purpose: To evaluate the influence of aerobic exercise on oxidative stress in mice. Methods: The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during 5 minutes for 3 consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialdehyde by the TBA method. The samples were read in a spectrophotometer at 535 nm. Results: The MDA intergroup analysis did not reveal significant increase when comparing the evaluated tissues. A significant difference was observed in the control group (p = 0.0201) during the intragroup comparison. Significant lower values were noticed in the smooth tissue than in plasma. The GB analysis indicated significant difference among the tissues (p= 0.0009), with lower values in the smooth muscle in relation to plasma (p <0.001) and higher in striated muscle in relation to smooth muscle (p <0.05),was observed. Conclusion: Physical exercise did not result in significant increase on the levels of oxidative stress in mice.