Use este identificador para citar ou linkar para este item: https://repositorio.ufms.br/handle/123456789/147
Tipo: Artigo de Periódico
Título: Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue
Autor(es): Berra, Carolina Maria
Menck, Carlos Frederico Martins
Martinez, Glaucia Regina
Oliveira, Carla Santos de
Baptista, Maurício da Silva
Di Mascio, Paolo
Abstract: To investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (1O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue. DNA lesions were detected by Fpg that cleaves DNA at certain oxidized bases, and T4-endoV, which cleaves DNA at cyclobutane pyrimidine dimers and apurinic/apyrimidinic (AP) sites. These cleavages form open relaxed-DNA structures, which are discriminated from supercoiled-DNA. DHPNO2 or photoactivated-MB treatments result in similar plasmid damage profile: low number of single-strand breaks or AP-sites and high frequency of Fpg-sensitive sites; confirming that base oxidation is the main product for both reactions and that 1O2 might be the most likely intermediate that reacts with DNA.
Palavras-chave: Estresse Oxidativo
Dano ao DNA
Oxidative Stress
Oxidative Stress
DNA Damage
Singlet Oxygen
Editor: Química Nova
Citação: BERRA, Carolina Maria et al . Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue. Quím. Nova, São Paulo, v. 33, n. 2, 2010 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000200009&lng=en&nrm=iso>. access on 20 July 2011. http://dx.doi.org/10.1590/S0100-40422010000200009.
Tipo de Acesso: Acesso Aberto
Identificador DOI: 10.1590/S0100-40422010000200009
URI: https://repositorio.ufms.br/handle/123456789/147
Data do documento: 2010
Aparece nas coleções:CCBS - Artigos publicados em periódicos

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