Use este identificador para citar ou linkar para este item: https://repositorio.ufms.br/handle/123456789/1278
Tipo: Artigo de Periódico
Título: Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
Autor(es): Araújo, Cristina Pires de
Leite, Clarice Queico Fugimura
Prince, Karina Andrade de
Jorge, Klaudia dos Santos Gonçalves
Osório, Ana Luiza Alves Rosa
Abstract: The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
Palavras-chave: Mycobacterium Bovis
Micobactérias não Tuberculosas
Reação em Cadeia da Polimerase
Bovinos
Matadouros
Nontuberculous Mycobacteria
Polymerase Chain Reaction
Cattle
Abattoirs
Editor: Memórias do Instituto Oswaldo Cruz
Citação: ARAUJO, Cristina Pires de et al . Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, Nov. 2005 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700013&lng=en&nrm=iso>. access on 19 Apr. 2012.
Tipo de Acesso: Acesso Aberto
Identificador DOI: http://dx.doi.org/10.1590/S0074-02762005000700013.
URI: https://repositorio.ufms.br/handle/123456789/1278
Data do documento: Nov-2005
Aparece nas coleções:CCBS - Artigos publicados em periódicos

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