ANÁLISE ESPECTROSCÓPICA (FTIR) DE CEPAS DE LEISHMANIA spp. PROVENIENTES DE CULTIVO AXÊNICO

Abstract

Leishmaniases are neotropical vector-borne infectious-parasitic diseases. These diseases primarily affect underdeveloped regions and are considered neglected, with a high incidence in Brazil. The isolation of Leishmania spp. strains based on indirect parasitological diagnosis allows for the identification of the species involved in the clinical case. This contributes to the surveillance and epidemiological control of the parasitosis, as well as directing clinical management towards a more effective therapeutic approach. The taxonomic characterization of the parasite is typically performed through the use of isoenzyme electrophoresis or molecular methods. These techniques, however, are considered laborious and are typically executed in reference laboratories. The application of Fourier-transform infrared spectroscopy (FTIR), in conjunction with multivariate data analysis utilizing algorithms, has been instrumental in the identification of microorganisms. The analysis of their infrared spectra offers insights into the vibrational profile of their components. This innovative method is characterized by its ease of application and high reproducibility, resulting in substantial precision, specificity, sensitivity, and low false positive and true negative error rates.