Use este identificador para citar ou linkar para este item: https://repositorio.ufms.br/handle/123456789/1070
Tipo: Artigo de Periódico
Título: Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil
Autor(es): Savani, Elisa San Martin Mouriz
Nunes, Vania Lúcia Brandão
Galati, Eunice Aparecida Bianchi
Castilho, Tiago Moreno
Araujo, Fernando Shiroma de
Ilha, Iêda Maria Novaes
Camargo, Maria Cecília Gibrail de Oliveira
D'Auria, Sandra Regina Nicoletti
Floeter-Winter, Lucile Maria
Abstract: A natural case of co-infection by Leishmania and Trypanosoma is reported in a dog (Canis familiaris) in south- western state of Mato Grosso do Sul, Brazil. Both amastigote and trypomastigote forms were observed after Giemsa staining of cytological preparations of the dog's bone marrow aspirate. No parasite was detected using medium culture inoculation of the sample. DNA obtained from the bone marrow aspirate sample and from the blood buffy coat was submitted to polymerase chain reaction (PCR) with a set of rDNA-based primers S4/S12. The nucleotide sequence of the PCR product was identical to that of Trypanosoma (Trypanozoon) evansi. The S4/S12 PCR was then used as template in a nested-PCR using a specific Leishmania set S17/S18 as primers, to explain the amastigote forms. The nucleotide sequence of the new PCR product was identical to that of Leishmania (Leishmania) chagasi. This case, as far as we know, is the first report of a dog co-infected with these parasites, suggesting that besides L. (L.) chagasi, the natural transmission of T. (T.) evansi occurs in the area under study.
Palavras-chave: Reação em Cadeia da Polimerase
Leishmania
Trypanosoma
Polymerase Chain Reaction
Editor: Memórias do Instituto Oswaldo Cruz
Citação: SAVANI, Elisa San Martin Mouriz et al . Ocurrence of co-infection by Leishmania (Leishmania) chagasi and Trypanosoma (Trypanozoon) evansi in a dog in the state of Mato Grosso do Sul, Brazil. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 100, n. 7, nov. 2005 . Disponível em <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762005000700011&lng=pt&nrm=iso>. acessos em 27 fev. 2012. http://dx.doi.org/10.1590/S0074-02762005000700011.
Tipo de Acesso: Acesso Aberto
Identificador DOI: http://dx.doi.org/10.1590/S0074-02762005000700011
URI: https://repositorio.ufms.br/handle/123456789/1070
Data do documento: 2005
Aparece nas coleções:CCBS - Artigos publicados em periódicos

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