Avaliação toxicogenética, bioquímica e da reparação tecidual em modelo experimental submetido a tratamentos com extratos de handroanthus impetiginosus (mart. ex dc) mattos (ipê-roxo) e de stryphnodendron adstringens (mart.) coville (barbatimão)

Abstract

Aim of the study: To evaluate the genotoxic, mutagenic, biochemical, and tissue repair effects in experimental model treated with ointments formulated from Handroanthus impetiginosus (HIWE-O) and Stryphnodendron adstringens (SAWE-O) extract. Materials and methods: Sixty Wistar rats were randomized in six groups. A standardized skin lesion was performed in the rats using a metal punch. After the performed skin lesion, groups 1 and 4 received a topical ointment base (solid Vaseline) daily for a period of 7 and 14 days, respectively, groups 2 and 5 received a topical application of HIWE-O at a concentration of 10% daily, for a period of 7 and 14 days, respectively, and t groups 3 and 6 received a topic application of SAWE-O at a concentration of10% daily for a period of 7 and14 days, respectively. Results: The chemical analysis of the SAWE profile revealed the presence of substances belonging to proanthocyanidins, prodelphinidins, and prorobinetinidis, and the chemical profile of Handroanthus impetiginosus revealed the presence of substances belonging to iridoid, glycoside, and phenylpropanoid glycoside classes. Histological findings revealed complete epithelialization by day 14 in all the animals treated topically with HIWE-O and SAWE-O, while only two animals in the control group had a completely epithelialized lesion during this period (Test: Chi-square (p<0.05)). The biological assays revealed no genotoxic and mutagenic effects of HIWE-O and SAWE-O. The biochemical analysis of HIWE-O and SAWE-O groups showed significant differences in GOT and TGP compared to the reference values (p<0,05; ANOVA/Tukey). Conclusions: The extracts of Handroanthus impetiginosus and Stryphnodendron adstringens susbtances presented with astringent action, anti-inflammatory and antioxidant, favored tissue repair of skin lesions, did not exhibit genotoxic and mutagenic effects and did not affect the evaluated biochemical parameters.