INDUÇÃO ANESTÉSICA COM ÓLEO ESSENCIAL DE MANJERICÃO E EUGENOL EM LAMBARI-DO-RABO-AMARELO (Astyanax lacustris): ESTRESSE E QUALIDADE SEMINAL

Abstract

The use of essential oils as anesthetics in fish has emerged as a promising strategy for reducing the impact of stressors on welfare and, consequently, performance during reproductive management. Therefore, although these procedures may help mitigate stress and mortality, there is little information regarding the effect of anesthesia on seminal quality. Therefore, this study aimed to evaluate the anesthetic effects of basil essential oil and eugenol on physiological stress and seminal quality in yellow-tailed lambari (Astyanax lacustris). One hundred and eight fish (14.38 g) were distributed in a completely randomized design with three treatments: (i) control; (ii) basil essential oil; and (iii) eugenol. Twenty-L aquariums were used as experimental units, with six replicates for each treatment. Each replicate consisted of one aquarium with six fish. The fish were induced with carp pituitary extract at a dosage of 2.5 mg kg-1 of body weight, in a single application. After 220-degree hours, semen was collected, forming two pools (replicates) per aquarium, each composed of the semen of three fish. The results obtained indicated that there was no significant difference (p> 0.05) in the seminal characteristics and survival rate of the yellow-tailed lambari after the use of anesthetics during reproductive management. However, it was observed that the blood glucose of the fish was higher (p<0.05) in the group anesthetized with basil essential oil (75.54 mg dL-1) followed by eugenol (69.14 mg dL-1) not differing significantly (p> 0.05) from the fish in the control group (61.46 mg dL-1). From the results found, it is concluded that the use of basil essential oil and eugenol were not able to mitigate the effects of stress on the yellow-tailed lambari during reproductive management but were efficient in preserving sperm quality.