PARÂMETROS DE TEMPERAMENTO E ANÁLISE METABOLÔMICA NA PROBABILIDADE DE PRENHEZ DE FÊMEAS BOVINAS EM IATF

Abstract

The aim of this study was to evaluate the metabolomic profile of Nelore females using 1H NMR technology to identify potential biological markers related to fertility and pregnancy. All females underwent a protocol of ovulation synchronization and fixed-time artificial insemination (FTAI). Gynecological parameters for the variables of ovarian follicle diameter (FD), uterine diameter (UT) and presence of corpus luteum (CL) were collected by transrectal ultrasonography. Reactivity was measured by visual assessment of the agitation score: movement (MV), trunk score (TS), tension (TS) and escape velocity (VF). Throughout the synchronization protocol, blood samples were collected for 1H NMR analysis. Through 1H NMR analysis, it was possible to identify 20 metabolites in the serum samples (n = 10), including amino acids, fatty acids and lipoproteins. Supervised chemometric analysis (sPLS-DA) revealed a distinction between the metabolic profiles of pregnant and non-pregnant females on the day of the synchronization protocol (D0) and on the day of pregnancy diagnosis (D40). Metabolites such as leucine, isoleucine, valine and alanine were identified as important in the separation of the groups, indicating their potential as fertility markers. In addition, very low-density and low-density lipoproteins (LDL/VLDL) and unsaturated fatty acids showed different levels between the groups, related to lipid metabolism and cholesterol transport, important for reproductive development. In contrast, on the day of artificial insemination (D10) there was no distinction between the groups evaluated. In summary, it is concluded that the biological markers found in the present study stand out for their role in lipid metabolism, cholesterol transport and reproductive development, indicating potential relevance in stages prior to artificial insemination to predict fertility in bovine females.