INFLUÊNCIA DA SUPLEMENTAÇÃO COM CREATINA E DO TREINAMENTO FÍSICO INTERVALADO SOBRE MARCADORES METABÓLICOS E INDICADORES DE REMODELAÇÃO MUSCULAR ESQUELÉTICA EM RATOS SAUDÁVEIS

Abstract

High-intensity interval training (HIIT) has been used in several studies because it provides metabolic and functional adaptations, contributing to different health benefits. Creatine is the most used ingredient among athletes, playing an important role in the energy metabolism of tissues that demand high energy production during effort and contributing to muscle mass gain. The objective of this work was to analyze the influence of creatine supplementation and HIIT on serum metabolic markers and tissue remodeling in different skeletal muscles of healthy rats. Male Wistar rats (n=40) were divided into four groups: Control (C), Creatine (Cr), Training (T) and Training and Creatine (TC). C and T groups were treated daily with standard commercial feed, while Cr and TC received feed + 2% creatine. The T and TC groups were submitted to a HIIT protocol consisting of five sessions per week for 12 weeks. Afterwards, nutritional characteristics, functional capacity, serum biochemical profile, morphological aspects as well as NAD and NADH expression from gastrocnemius (white portion) and soleus muscles were analyzed. The results were analyzed using Tukey's two-way complemented ANOVA. Food intake, caloric intake and caloric efficiency were lower in trained than sedentary control groups. HIIT was associated with lower values of body mass and adiposity, as well as reduced levels of total cholesterol and triglycerides. In the effort test, distance and maximum speed were increased in response to training. Independently, respective relationships between gastrocnemius and soleus masses with body mass were increased with HIIT. Creatine supplementation reduced NADH expression, as well as NAD and NADH in collagen association in the gastrocnemius, which was accentuated in the presence of HIIT. Although it did not affect morphological aspects of the skeletal muscles, the combination of HIIT and creatine resulted in greater effort performance and reduced the expression of reduced NADH, NAD and NADH in collagen association in the gastrocnemius muscle (white portion), increased the NADH/NAD ratio and interstitial collagen remodeling in the soleus muscle.