DESENVOLVIMENTO E TESTAGEM DE UM PROTÓTIPO DE BIOCURATIVO DE LIBERAÇÃO CONTROLADA ENRIQUECIDO COM CÉLULAS-TRONCO MESENQUIMAIS

Abstract

To date, there is no gold standard for treating burns. There is no consensus in the literature on the subject and a large number of strategies are used with good results. Among these, the use of sodium alginate dressing and the transplantation of mesenchymal stem cells stand out. In the present proposal, we combine these two technologies in the search for better results. Aiming to develop and test the prototype of a controlled release biodressing enriched with mesenchymal stem cells (BioRelControl-MSC) in burns. The experiments were carried out with the cells of a donor. And they were performed in vitro and the culture plate was used as a mimic of the wound bed. The results indicated that BioRelControl-MSC does not cause DNA damage since it did not change the frequency of comets or the expression of the mTor, GADD45α, ATM, ATR and TP53 genes, as well as it does not change cell viability or gene expression. BAK, BAX, BBC3 and BCL-2. Cells migrate from the 3rd experimental day to the 50th. The migration peak occurs on the 36th day and at that moment there was an increase in the expression of the MMP1 and MMP9 genes, which are genes related to cell migration and adhesion. BioRelControl-MSC causes cell cycle retention in G1, however, it does not alter the expression of CHK1, CHK2, CDKN1α and NFKβ, thus, it is suggested that this retention may be related to the senescence process. This fact is confirmed by the reduction in telomere length that occurred over the experimental days, and the consequent increase in biological age. This assertion also corroborates the fact that there is an increase in oxidative stress on the 36th and 50th experimental days, which coincide with the worst biological ages. In view of the above, it is considered that the BioRelControl-MSC should be prepared and used in the first days since the storage of the product can increase biological age and oxidative stress. It is suggested that BioRelControl-MSC has appeal to become a product with clinical application in burn patients, however, more in vivo research is needed to prove its effectiveness and controlled release of mesenchymal stem cells.

Mesh: cell therapy; stem cells; sodium alginate; biocurative; burns.