Efeito da 7ß-hidroxietilteofilina e 8- fenilteofilina na aquisição e retenção da memória espacial em camundongos

Abstract

Introduction: Neurodegenerative diseases have a growing prevalence among the population over 65 years and adenosine antagonists have a neuromodulator role, especially in the hippocampus region, contributing to the maintenance and improvement of memory, with promising drugs for preventive medicine studies of complications neurodegenerative diseases. Objective: To evaluate the effect of different concentrations of 78-hydroxyethyltheophylline and 8- phenyltheophylline, acquisition and retention of spatial memory in mice. Methodology: Used swiss mice, males, 25-30g, subdivided in two experiments for each experiment were two application times, before and after the section of the training/test. The mice were subjected to training in the Water Maze Morris - LAM for four days (three sessions / day) and subjected to the test after 48 h from the last training session, and was measured the time for each animal to find the hidden platform. The animals of the first group (eight animals per dose of the drug) respectively received saline (SF) 10ml / kg, 78- hydroxyethyltheophylline at a dose of 10, 20 and 40 mg/kg 30 minutes before training intraperitoneally (ip). The second group used the same drug and the same dosages of the first group immediately after training. The third group received SF 10ml/kg, 8-phenyltheophylline at a dose of 1, 2 and 4 mg / kg ip 30 minutes before training. The fourth group received the same drug dosage and the third group immediately after training. The animals were euthanized in CO2 camera. The results were analyzed considering a 5% significance level by ANOVA two-way repeated measures, followed by Tukey's post-test. Results: The drugs did not reduce the latency for the animal to find the platform: Group I (p=0,089), II (p=0,641), III (p=0,089) e IV (p=0,290) with significant effect on the day of training/test: Group I (p<0,001), II (p<0,02), III (p<0,001) e IV (p<0,001). Conclusion: It is concluded that LAM is a useful test for assessing the acquisition and retention of spatial memory in mice and tested the experimental drug had no effect on spatial memory, independent of dose and time of application.