Efeitos do dimetilsulfóxido e da oxigenoterapia hiperbárica em retalho cutâneo: avaliação macroscópica e celular

Abstract

Purpose:This study aimed to investigate the effects of hyperbaric oxygen therapy and dimethyl sulfoxide (DMSO) in tissue necrosis, genotoxicity and cell apoptosis in random skin flaps. Method: Fifty male Wistar rats were randomly divided into five groups: control group (CT), where the retail rectangular skin (2x8cm) was dissected from the dorsal muscle layer, preserving the cranial vessels, high and fixed to the bed. Group distilled water (AD), AD injected himself in the distal half of the flap, as well as dimethyl sulfoxide group (DMSO) where 5% DMSO was injected, the HBOT group where it was held only treatment with HBO and HBO + DMSO group added treatment with 100% oxygen at 2,5 atmospheres absolute (ATA) for an hour, two hours after the experiment, daily for ten consecutive days. On the tenth day, the animals were photographed and performed at the central portion of tissue collected from distal flap viable for microscopic evaluation. Results: The percentage of necrosis at the end of the experiment the groups were: CT = 43,83 %, AD= 46,79 %, DMSO = 47,19 % OHB = 38,30% and DMSO + OHB = 45,94 % with p= 0,249 . The average weight gain between groups at the end of the experiment was 22,98 g with p= 0,266 We found a cell viability index of 87,40% (CT), 86,20% (AD), 84,60 % (DMSO), 86,60 % (DMSO + OHB) and 91% (OHB) with p< 0,001, and cell viability index of 12.60 (CT), 12.00 (AD), 15.40 (DMSO), 9.00 (OHB) and 12,00 (DMSO + OHB) with p< 0,001 genotoxicity test found a percentage of cells with DNA damage 22,80 (CT), 22,60 (AD), 26,00 (DMSO), 8,80 (DMSO + OHB), and 7,20 (OHB), with p <0,001. Conclusion: There was statistically significant effect of reduced area of necrosis between groups but there was a reduction in the cellular DNA damage and apoptosis index in the OHB group, statistically significant.