Estudo quantitativo na expressão de FAS ligante pós indução de estresse oxidativo em mucosa gástrica com administração de azoximetano, em ratos

Abstract

This experimental study quantified FAS ligand (FAS-L) expression on gastric mucosa of rats submited to Azoxymethane (AOM). Methods: Wistar rats (n=56) distributed in 2 groups: G1 (n=28) and G2 (n=28), submited to placebo (saline solution, 1 mL/Kg) or AOM (5 mg/Kg), respectively, in 3nd and 4th weeks. Euthanasia and gastrectomy 3 hours after injections, weekly, obteining four subgroups from each group: E1 (n=7), E2 (n7), E3 (n=7), E4 (n=7). Imunohistochemistry, digital capture and computer assisted quantitative processing with Adobe Photoshop™. Statistical analysis by Mann Wittman method, with significative increasing of FAS ligand expression percentage area in G2 (G1= 20,56 x G2= 27,66 and p<0.05) and decreasing gray scale optical density meaning increase of positive color (G1= 151,69 x 140,31 e p<0.01), however not significative diference in subgroups E2 e E4 positive area percentage (G1E2= 26,02 x G2E2= 27,40 and p=0,28; G1E4= 26,77 x G2E4= 26,77 and p= 0,33) and gray scale (G1E2= 142,03 x G2E2= 141,45 and p= 0,33; G1E4= 149,65 x G2E4= 141,02 and p=0,09). Conclusion: oxidative stress by AOM subcutaneous injection increases FAS-L expression in gastric mucosa, in rats.