Utilização de marcadores ISSR para identificação molecular de isolados ambientais de Aspergillus.

Abstract

Aspergillus is a widely distributed genus of filamentous fungi with significant ecological, economic, and medical relevance due to its adaptability to various environments and the pathogenic potential of certain species. This study evaluated the effectiveness of ISSR markers in the molecular identification of cryptic Aspergillus species, using 12 samples collected from 11 Latin American countries as part of the LatAsp project. Techniques such as MALDI-TOF, genetic sequencing, and PCR were employed. Although ISSR markers revealed species diversity, their specificity was insufficient to discriminate cryptic species, grouping isolates from different taxonomic sections and even distinct genera, such as Penicillium. These results emphasize the phylogenetic complexity of Aspergillus and the need for more accurate molecular methods and genetic markers. The findings highlight the importance of advancements in species identification, particularly in clinical contexts, to support effective treatment of aspergillosis.