Torção do cordão espermático: isquemia e reperfusão, avaliação toxicogenética e dos efeitos da fosfatidilcolina em ensaio pré-clínico

Abstract

Phosphatidylcholine is the main phospholipid present in cell membranes and in lipoproteins and thus is able to interfere in various biological processes. This lipid also has antioxidant activity, indicating protection from damage caused by free radicals under conditions of ischemia/reperfusion. Given the above, the present study aimed to evaluate toxicogenetic damage caused by twisting of the spermatic cord in ischemia/reperfusion and also if phosphatidylcholine plays a hole in ischemia/reperfusion condition in preclinical trials. The results indicate that the spermatic cord torsion does not cause genotoxic damage nor mutagenic. The phosphatidylcholine at the dose of 300mg / kg is toxic and thus it’s not recommended to use. However, the dose of 150mg / kg does not promote toxicogenetic damage and does not statistically prevent tissue damage occoured from lack of oxygenation and nutrition of testicular cells, although there was a tendency to reduce them. Therefore this research suggests that further studies should be conducted to clarify this tendency and also, to provide a better explanation on the possible therapeutic effects of phosphatidylcholine in cytoprotection of germ cells submitted to to ischemia/reperfusion.