https://repositorio.ufms.br/handle/123456789/6158 2026-04-18T18:42:24Z https://repositorio.ufms.br/handle/123456789/14295 Título: Relação entre a deficiência da enzima glicose-6-fosfato-desidrogenase (G6P-D) e o Transtorno do Espectro Autista (TEA): Revisão Integrativa. Abstract: Relationship between G6P-D deficiency associated with other developmental factors of ASD, such as oxidative stress, through an integrative review of the current scientific literature (2021 - 2026) Tipo: Trabalho de Conclusão de Curso 2026-01-01T00:00:00Z https://repositorio.ufms.br/handle/123456789/14050 Título: POTENCIAL BIOTECNOLÓGICO DO FUNGO ENDOFÍTICO Penicillium cataractarum 5IP2F4 NA PRODUÇÃO DE METABÓLITOS Abstract: Endophytic fungi are symbiotic microorganisms that inhabit plant tissues without causing damage. They produce secondary metabolites for defense and adaptation, making them an important source of natural products with biotechnological potential for the development of new drugs. Currently, the search for new antibacterial drugs is crucial due to the increasing bacterial resistance to available antibiotics. In this context, preliminary studies conducted at LaPNEM identified a promising strain, Penicillium cataractarum 5IP2F4, which demonstrated potential for action against the bacterium Staphylococcus aureus. In light of this, the present work aimed to cultivate the endophyte P. cataractarum 5IP2F4, previously isolated from Bromelia balansae collected in the Pantanal, using the One Strain Many Compounds (OSMAC) strategy and annotating the compounds present in the extracts. Chemical characterization of crude extracts obtained from the supernatants (sCE) and mycelial biomass/mycelia (mCE) of liquid culture was performed in the following media: potato-dextrose broth (PDB), commercial PDBc, yeast-peptone-dextrose extract (YPD), and Sabouraud (SAB), in search of new molecules, whose chemical profiles were analyzed by high-performance liquid chromatography coupled to a diode array detector and mass spectrometry. Compounds from the extracts were noted, such as coumarins tetrahydroxy-methyl-dihydroisocoumarin and trihydroxy-methyl-dihydroisocoumarin, which were produced in greater quantities in potato media, in addition to the cyclopeptide desferrichrome, produced by the mycelia in all 4 media, as well as citric acid, tyrosine, and adenosine. The YPD medium showed the highest yields of extracts and biomass, indicating more favorable nutritional conditions for growth, while metabolite production was more stimulated in potato media, even with a simpler composition. The chromatograms obtained from the extracts revealed different peaks between the media; furthermore, it was possible to observe a greater production of compounds by the fungus in the sCE compared to the mCE. With these results, the research also allowed for a greater understanding of the species, as there is insufficient data on P. cataratarum, as well as on the chemical composition of the endophyte community in the southern Pantanal region of Mato Grosso do Sul, highlighting the importance of conserving the biome. Tipo: Trabalho de Conclusão de Curso 2025-01-01T00:00:00Z https://repositorio.ufms.br/handle/123456789/13891 Título: OTIMIZAÇÃO DA PRODUÇÃO DE COMPOSTOS ANTIBACTERIANOS A PARTIR DO FUNGO ENDOFÍTICO Penicillium cataractarum 5IP2F4 Abstract: Endophytic fungi are an important source of secondary metabolites with potential pharmacological applications, standing out as promising candidates in the search for new antimicrobial compounds. Penicillium cataractarum is a species that remains poorly explored but it has shown relevant results in preliminary antibacterial studies. In this context, the present work aimed to investigate and optimize the production of antibacterial metabolites from the endophytic strain P. cataractarum 5IP2F4, isolated from Bromelia balansae collected in the s Pantanal of Mato Grosso do Sul (Brazil). The OSMAC (One Strain Many Compounds) strategy was applied by varying liquid culture media in order to modulate biosynthetic pathways and expand the chemical diversity produced by the strain. Antibacterial activity was assessed by broth microdilution assays, and chemical analyses of the extracts were performed using HPLC-DAD-MS and GC-MS. Variations among the culture media significantly influenced mycelial growth and the chemical profile of the extracts, resulting in differences in extract yields. Kinetic analysis indicated that day 22 corresponded to the point of highest accumulation of extracellular metabolites, reflected by the intensification of the four major annotated compounds, all classified as diketopiperazines. GC-MS analysis revealed marked variation among the media, with ergosterol predominating in the hexane fractions from mycelial extracts obtained in PDB and YPD cultures, while CZA medium favored the production of unsaturated fatty acid esters, especially methyl linoleate and methyl oleate. Regarding antibacterial inhibition, although none of the extracts displayed significant activity against Escherichia coli (ATCC 14458), the supernatant extract obtained from Czapek medium (EBsob–CZA) inhibited 68% of Staphylococcus aureus ATCC 25904 growth at 1 mg/mL. The findings reinforce that P. cataractarum 5IP2F4 possesses a versatile metabolome strongly dependent on culture conditions, confirming the importance of the OSMAC strategy as a tool for activating biosynthetic pathways and expanding accessible chemical diversity. Overall, this study contributes to the understanding of the biosynthetic potential of P. cataractarum and highlights its relevance as an alternative source of metabolites with antibacterial activity, particularly against pathogens of public health importance. Tipo: Trabalho de Conclusão de Curso 2025-01-01T00:00:00Z https://repositorio.ufms.br/handle/123456789/13890 Título: ANÁLISE DO ÓLEO ESSENCIAL DE ELIONURUS MUTICUS (SPRENG.) KUNTZE POR CROMATOGRAFIA EM FASE GASOSA ACOPLADA À ESPECTROMETRIA DE MASSAS (CG-EM) Abstract: Elionurus muticus (Spreng.) Kuntze, a grass species belonging to the family Poaceae, occurs in tropical and subtropical regions and is popularly known as “capim carona”. Traditionally, it has been used for its diaphoretic and antipyretic properties, although its full potential and characteristics are not yet completely known. In this study, the chemical profile of the essential oil of E. muticus was analyzed, along with the total ash content and loss on drying. The leaves were collected in the Nhecolândia region (Mato Grosso do Sul, Brazil) and identified by the botanist prof. Dr. Geraldo Alves Damasceno Junior. The essential oil was extracted by hydrodistillation using a Clevenger apparatus, yielding 0.67% ± 0.09% and its chemical composition was determined by gas chromatography coupled to mass spectrometry (GC-MS). A total of 22 compounds were noted and the major constituents were (Z)-caryophyllene (27.93%), spathulenol (24.37%), caryophyllene oxide (15.17%) and bicyclogermacrene (8.23%). The mean total ash content was 3.56 ± 0.68%, while the humidity was 8.00 ± 0.81% determined by loss on drying. The results showed that the essential oil of E. muticus has a composition rich in hydrocarbon and oxygenated sesquiterpenes, highlighting the potential of the species, as well as contributing to the chemical and ecological understanding of the Pantanal biome in southern Mato Grosso do Sul. Tipo: Trabalho de Conclusão de Curso 2025-01-01T00:00:00Z