DSpace Coleção:https://repositorio.ufms.br/handle/123456789/36072026-03-27T22:19:49Z2026-03-27T22:19:49ZDesenvolvimento e avaliação da atividade anti-inflamatória da nanoemulsão do óleo dos frutos de Acrocomia aculeatahttps://repositorio.ufms.br/handle/123456789/142272026-01-22T15:09:24Z2025-01-01T00:00:00ZTítulo: Desenvolvimento e avaliação da atividade anti-inflamatória da nanoemulsão do óleo dos frutos de Acrocomia aculeata
Abstract: Acrocomia aculeata Jacq. is a species of the Arecaceae family, commonly known as bocaiuva or macaúba. It is widespread in South America, particularly in the state of Mato Grosso do Sul. Acrocomia aculeata oil (AAO) is traditionally used by communities in the Central-West region of Brazil to treat various conditions, including inflammation, excess mucus, and skin lesions. Despite its therapeutic potential, research exploring its composition, properties, and possible applications is still limited. Nanoencapsulation of vegetable oils is an innovative technique that improves the bioavailability and stability of these inputs. This study aimed to develop and evaluate the anti-inflammatory activity of Acrocomia aculeata oil-loaded nanoemulsion (AANE). The chemical characterization of AAO was performed by gas chromatography coupled to mass spectrometry (GC/MS). Subsequently, a nanoemulsion was formulated using the phase inversion method, employing non-ionic surfactants to ensure the stability of the colloidal system. The composition of the AAO revealed a high content of polyphenols (12.60 mg/100g) and carotenoids (266.00 mg/100g), with a predominance of monounsaturated fatty acids (74%), of which oleic acid represents 71.25%, characteristics that favor its antioxidant and anti-inflammatory profile. The developed AANE presented droplets size of 173.6 ± 0.70 nm, a polydispersity index of 0.200, with a zeta potential of −14.10 ± 1.06 mV. These parameters remained constant for 180 days at 25 ± 2 °C on a shelf. The nanoemulsion showed excellent anti-inflammatory activity in a carrageenan-induced inflammation model (paw edema) in mice. The nanoemulsion showed no cytotoxic effects in the cell viability assay, which presented 100% survival at all concentrations tested (1, 10, 100, and 1000 μg/mL). Regarding biological safety, the nanoemulsion did not show hemolytic activity, even at high concentrations (up to 1000 μg/mL), contrasting with the positive control TritonX-100, which induced significant hemolysis, thus suggesting a safe profile for therapeutic application in preclinical models. This study enabled the development and characterization of Acrocomia aculeata oil-loaded nanoemulsion with promising anti-inflammatory effects and absence of toxicity. This product not only expands the applications of bocaiuva oil in the fields of health and biotechnology, but also adds value to a native plant resource from the Central-West region of Brazil, with potential for future applications in the development of phytopharmaceuticals.
Palabras clave: Bocaiuva, nanoemulsion, anti-inflammatory, nanotechnology, toxicity.
Tipo: Tese2025-01-01T00:00:00ZAvaliação da atividade antileishmania in vitro de compostos imidazo[2,1-b]tiazóis sintéticoshttps://repositorio.ufms.br/handle/123456789/142002026-01-14T01:41:39Z2025-01-01T00:00:00ZTítulo: Avaliação da atividade antileishmania in vitro de compostos imidazo[2,1-b]tiazóis sintéticos
Abstract: Leishmaniases are neglected tropical diseases for which the current treatments are highly toxic, require long-term administration and are costly. They are also subject to parasitic resistance. The cutaneous form is the most prevalent, particularly in South American countries such as Brazil. Therefore, developing safer and more effective therapies is essential. In this regard, synthetic heterocyclic compounds containing selenium and sulphur atoms exhibit promising antileishmania activity. The aim of this project was to investigate the in vitro antileishmania activity of synthetic compounds containing selenium and sulfur with imidazo[2,1-b]thiazole structures. The compounds were tested against promastigotes and intracellular amastigotes of Leishmania amazonensis, and their cytotoxicity was evaluated in a mouse fibroblast cell line. The compounds exhibited activity against the promastigote forms of L. amazonensis with minimal reduction in NIH/3T3 fibroblast viability, and some demonstrated activity against the intracellular amastigote forms. Compound S61 exhibited the greatest activity against intracellular amastigote forms, demonstrating an IC₅₀ of 5.66 μM and a selectivity index of 17.9. Most of the compounds exhibited desirable physicochemical properties that are compatible with the development of new drugs. The results of this project made it possible to screen promising compounds that will be evaluated in future in vitro and in vivo studies for their potential molecular targets, with the aim of developing new therapeutic alternatives for leishmaniasis.
Tipo: Dissertação2025-01-01T00:00:00ZDesenvolvimento de kefir de leite e soro de búfala e caracterização como alimento funcionalhttps://repositorio.ufms.br/handle/123456789/124112025-09-25T14:57:45Z2025-01-01T00:00:00ZTítulo: Desenvolvimento de kefir de leite e soro de búfala e caracterização como alimento funcional
Tipo: Dissertação2025-01-01T00:00:00ZSalvinia auriculata como uma fonte inovadora de metabólitos bioativoshttps://repositorio.ufms.br/handle/123456789/119852025-06-25T14:31:22Z2025-01-01T00:00:00ZTítulo: Salvinia auriculata como uma fonte inovadora de metabólitos bioativos
Abstract: Salvinia auriculata is a free-floating aquatic macrophyte widely distributed in
tropical and subtropical regions, known for its rapid growth, invasive potential, and
production of secondary metabolites with diverse bioactivities. Although traditionally
studied for its phytoremediation potential, the species has shown promising
biotechnological applications, particularly in pharmaceutical and veterinary fields, due
to the presence of compounds such as salvinisides and flavonoids. Samples were
collected from the Lagoa Comprida Municipal Natural Park (Mato Grosso do Sul,
Brazil), dehydrated, ground, and subjected to two extraction methods: percolation and
pressurized fluid extraction (PFE), yielding 8.7% and 7.64%, respectively. The
resulting fractions were analyzed by HPLC-DAD-MS/MS and submitted to quantitative
determinations of phenolic compounds and tannins. Biological evaluations included
assays for anthelmintic, acaricidal, and anti-Leishmania activities, using Haemonchus
contortus, Rhipicephalus spp., and Leishmania amazonensis. Chemical analysis
revealed high contents of total phenolics (6.25% GAE) and tannins (9.85%), in addition
to the detection of compounds such as isomers of salviniside II, previously described
in the literature but still scarcely investigated regarding their biological activity. The
tested extracts and fractions exhibited varying levels of efficacy across the bioassays,
including moderate inhibition of larval migration, selective acaricidal activity, and
cytotoxic effects against Leishmania. The ethyl acetate fraction showed the highest
antioxidant and anti-Leishmania activity, while the crude extract exhibited the most
prominent acaricidal effects. The findings confirm the potential of Salvinia auriculata
as a source of bioactive metabolites with therapeutic and veterinary relevance. The
chemical diversity and bioactivity observed in the tested fractions support the
continuation of research focused on the isolation and characterization of active
compounds and the development of formulations for parasite control and the
sustainable use of invasive aquatic species.
Tipo: Tese2025-01-01T00:00:00Z