Use este identificador para citar ou linkar para este item: https://repositorio.ufms.br/handle/123456789/1411
Tipo: Artigo de Periódico
Título: Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children
Autor(es): Fraga, Thiago Leite
Brustoloni, Yvone Maia
Lima, Rosimar Baptista
Dorval, Maria Elizabeth Cavalheiros
Oshiro, Elisa Teruya
Oliveira, Janaina
Oliveira, Ana Lúcia Lyrio de
Pirmez, Claude
Abstract: The diagnosis of visceral leishmaniasis (VL) generally requires the use of invasive tests for the collection of infected tissue (aspirates of bone marrow, spleen, liver or lymph nodes). This difficulty has led to the search for safer and less painful techniques to confirm the occurrence of the disease in children. Polymerase chain reaction (PCR) is a method that is advantageous in that it allows the use of peripheral blood samples for diagnosis. This paper reports the utilisation of PCR on peripheral blood samples to diagnose VL in 45 children in Mato Grosso do Sul, Brazil. This technique is compared with methods carried out using tissue collected by invasive procedures, including direct microscopy, culture and detection of Leishmania DNA by PCR in bone marrow aspirates. The results show that PCR of peripheral blood provides great sensitivity (95.6%) that is similar to that from the PCR of bone marrow aspirates (91.1%) and higher than that achieved with microscopy (80%) or culture (26.7%) methods. PCR of peripheral blood proved to be a suitable tool for the diagnosis of VL in children because it is highly sensitive and safe, with tissue collection being less invasive than in traditional tests.
Palavras-chave: Leishmaniasis
Reação em Cadeia da Polimerase
Sangue
Criança
Polymerase Chain Reaction
Blood
Child
Editor: Memórias do Instituto Oswaldo Cruz
Citação: FRAGA, Thiago Leite et al . Polymerase chain reaction of peripheral blood as a tool for the diagnosis of visceral leishmaniasis in children. Mem. Inst. Oswaldo Cruz, Rio de Janeiro, v. 105, n. 3, May 2010 . Available from <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300011&lng=en&nrm=iso>. access on 22 May 2012.
Tipo de Acesso: Acesso Aberto
Identificador DOI: http://dx.doi.org/10.1590/S0074-02762010000300011.
URI: https://repositorio.ufms.br/handle/123456789/1411
Data do documento: Mai-2010
Aparece nas coleções:FAMED - Artigos publicados em periódicos

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