https://repositorio.ufms.br/handle/123456789/6158 Fri, 19 Jun 2026 13:31:07 GMT 2026-06-19T13:31:07Z https://repositorio.ufms.br/handle/123456789/14457 Título: DESAFIOS ESTRUTURAIS NO MINOXIDIL: TAUTOMERISMO IMINA-ENAMINA Abstract: Minoxidil is a vasodilator drug widely used in the treatment of hair loss, whose chemical structure in solution still presents poorly explored aspects, with possible impact on spectral interpretation and on the quantification of the active compound in pharmaceutical formulations. This study aimed to investigate structural characteristics of minoxidil in solution by means of 1H and 13C Nuclear Magnetic Resonance (NMR) spectroscopy. Initially, solubility tests were performed in different solvents, with tetradeuterated methanol (CD3OD-d4) being the medium that showed the best performance for the analysis. In the experiments conducted in CD₃OD-d4, a selective reduction in the integral of the aromatic hydrogen was observed over time, with greater intensity at 25 ºC than at 5 ºC; Additionally, the progressive emergence of a triplet in the 13C NMR spectra, compatible with C-D coupling was observed. In contrast, in the experiments performed in hexadeuterated dimethyl sulfoxide (DMSO-d₆), the proportion between the integrals of the 1H NMR signals remained stable for approximately 59 h, and the 13C NMR spectra did not show the emergence of the triplet or other relevant changes. Taken together, the results indicate that the behavior of minoxidil in solution is strongly dependent on the solvent and temperature, being compatible with the occurrence of imine-enamine tautomerism and H/D exchange in a deuterated protic solvent, providing support for more consistent structural interpretations and for the development of analytical methodologies applied to quality control and quantification of this drug. Tipo: Trabalho de Conclusão de Curso Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ufms.br/handle/123456789/14457 2026-01-01T00:00:00Z https://repositorio.ufms.br/handle/123456789/14438 Título: Análise do perfil farmacoterapêutico e das potenciais interações medicamentosas em pacientes com Esclerose Múltipla Abstract: Multiple sclerosis (MS) is an immune-mediated, inflammatory, demyelinating, and neurodegenerative disease that primarily affects young adults between 20 and 50 years of age, with a higher prevalence in women. Patients with MS commonly present with symptoms resulting from the disease itself and other associated comorbidities. Therefore, drug treatment aims at clinical improvement, increased functional capacity, improved prognosis, and symptom attenuation; consequently, these patients are subject to polypharmacy. The simultaneous use of multiple drugs is associated with an increased likelihood of the patient experiencing drug-related problems (DRPs), which can compromise the effectiveness of pharmacological treatment and/or generate health problems. Harmful drug interactions are examples of DRPs and therefore must be identified to ensure adequate treatment and patient safety. The objective of this study is to analyze the medication use profile, prevalence, and potential drug interactions in patients with multiple sclerosis undergoing treatment with natalizumab, as seen at the Pharmacy School of the Federal University of Mato Grosso do Sul (UFMS). To this end, a cross-sectional observational study was conducted with 60 patients treated with natalizumab at the institution. The study showed evidence of a link between a complex pharmacotherapy regimen and the occurrence of drug-related problems (DRPs). Factors such as the total number of medications in use, the presence of comorbidities, and polypharmacy are directly associated with an increased risk of potential drug interactions. The study also observed that the classes of psychoanaleptics, antiepileptics, and psycholeptics had a greater impact on the studied interactions. Although most complementary therapies have not been shown to be involved in potential interactions, some supplements have been associated with clinically relevant combinations, such as that observed between magnesium supplementation and vitamin D use. Finally, the importance of the pharmacist's role in managing these DRPs and promoting health by contributing with interventions and services is highlighted. Tipo: Trabalho de Conclusão de Curso Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ufms.br/handle/123456789/14438 2026-01-01T00:00:00Z https://repositorio.ufms.br/handle/123456789/14295 Título: Relação entre a deficiência da enzima glicose-6-fosfato-desidrogenase (G6P-D) e o Transtorno do Espectro Autista (TEA): Revisão Integrativa. Abstract: Relationship between G6P-D deficiency associated with other developmental factors of ASD, such as oxidative stress, through an integrative review of the current scientific literature (2021 - 2026) Tipo: Trabalho de Conclusão de Curso Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ufms.br/handle/123456789/14295 2026-01-01T00:00:00Z https://repositorio.ufms.br/handle/123456789/14050 Título: POTENCIAL BIOTECNOLÓGICO DO FUNGO ENDOFÍTICO Penicillium cataractarum 5IP2F4 NA PRODUÇÃO DE METABÓLITOS Abstract: Endophytic fungi are symbiotic microorganisms that inhabit plant tissues without causing damage. They produce secondary metabolites for defense and adaptation, making them an important source of natural products with biotechnological potential for the development of new drugs. Currently, the search for new antibacterial drugs is crucial due to the increasing bacterial resistance to available antibiotics. In this context, preliminary studies conducted at LaPNEM identified a promising strain, Penicillium cataractarum 5IP2F4, which demonstrated potential for action against the bacterium Staphylococcus aureus. In light of this, the present work aimed to cultivate the endophyte P. cataractarum 5IP2F4, previously isolated from Bromelia balansae collected in the Pantanal, using the One Strain Many Compounds (OSMAC) strategy and annotating the compounds present in the extracts. Chemical characterization of crude extracts obtained from the supernatants (sCE) and mycelial biomass/mycelia (mCE) of liquid culture was performed in the following media: potato-dextrose broth (PDB), commercial PDBc, yeast-peptone-dextrose extract (YPD), and Sabouraud (SAB), in search of new molecules, whose chemical profiles were analyzed by high-performance liquid chromatography coupled to a diode array detector and mass spectrometry. Compounds from the extracts were noted, such as coumarins tetrahydroxy-methyl-dihydroisocoumarin and trihydroxy-methyl-dihydroisocoumarin, which were produced in greater quantities in potato media, in addition to the cyclopeptide desferrichrome, produced by the mycelia in all 4 media, as well as citric acid, tyrosine, and adenosine. The YPD medium showed the highest yields of extracts and biomass, indicating more favorable nutritional conditions for growth, while metabolite production was more stimulated in potato media, even with a simpler composition. The chromatograms obtained from the extracts revealed different peaks between the media; furthermore, it was possible to observe a greater production of compounds by the fungus in the sCE compared to the mCE. With these results, the research also allowed for a greater understanding of the species, as there is insufficient data on P. cataratarum, as well as on the chemical composition of the endophyte community in the southern Pantanal region of Mato Grosso do Sul, highlighting the importance of conserving the biome. Tipo: Trabalho de Conclusão de Curso Wed, 01 Jan 2025 00:00:00 GMT https://repositorio.ufms.br/handle/123456789/14050 2025-01-01T00:00:00Z